Sheng Du, Chen Gong, De-Zhi Meng, Ze-Hua Mou,
Xi-Feng Cui, Fang-Zheng Li
College of Animal Science and Technology, Qingdao Agricultural University，Qingdao, 266109, China
Fang-Zheng Li (E-mail: firstname.lastname@example.org)
Anatomy; Clinical examinations; Hematology; Histology; Internal medicine; Veterinary medicine
Preparation of transparent and stained casting
specimens is useful in accurately understanding the vessels and segments in
organs. In this study, we prepared epoxy resin of different color and filled
the resin into hepatic portal veins and hepatic veins of fresh rabbit livers.
Then the livers were treated for transparency serially using formalin, hydrogen
dioxide, gradient alcohol, potassium hydroxide, and glycerol. The resultant
transparent stained casting live specimens clearly showed the distribution of
internal vessels and external structures of hepatic portal veins and hepatic
veins. This method can be also applied for preparation of transparent and
stained casting specimens of other organs for education and research.