Newpubli (ISSN 2474-8242) is the first fifth-generation scientific journal

Newpubli provides a value rank for each article through peer review

Effect of Low-Concentration of Copper on Virulence Factors of a Pathogenic Escherichia Coli Strain

Yang Yang, Yun Liu, Qiumei Shi, Zhiqiang Zhang, Tonglei Wu, Guoqiang Zhu
Author Affiliation(s)
Jiangsu Co-Innovation Centerfor for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, College of Veterinary Medicine, Yangzhou University, Yangzhou, 225009, China (Yang Yang, Yun Liu, Guoqiang Zhu); Hebei Normal University of Science & Technology, Key Laboratory of Hebei Province Preventive Veterinary Medicine, Qinhuangdao, 066004, China (Qiumei Shi, Zhiqiang Zhang, Tonglei Wu)
Corresponding Author(s)
Guoqiang Zhu (E-mail:,
Subject Areas
Bacteriology; Infectious diseases; Inorganic chemistry; Microbiology; Pharmacology; Veterinary medicine

Low-concentration dietary copper has been widely used for domestic animals worldwide. To investigate the potential antimicrobial effect of low-concentration of copper, a pathogenic Shiga-like toxigenic Escherichia coli (SLTEC) strain, 107/86, cultured in the medium complemented with 1.0 mmol/L copper, was examined regarding alteration of certain virulence factors. The results showed that, compared to the untreated control strain, the Cu-treated strain 107/86 displayed suppressed growth rate, impaired adherence to IPEC-J2 cells, and decreased stx2e production. Reduced adherence to IPEC-J2 cells was confirmed using a real-time RT-PCR assay which targeted adhesion-associated genes. Additionally, the Cu-treated 107/86 differed from the untreated control with 33-fold lower activity of AI-2 in the quorum sensing system as detected using a luminescence assay. Less biofilm formation and decreased flagella motility halos were detected in the Cu-treated strain. Taken together, low-concentration of copper in the medium likely led to significant attenuation of multiple virulence factors of the SLTEC strain, providing new insights to the antimicrobial effect of copper.

Full Text
Hyperlinks of the full text in HTML, PDF and DOC are available at the right side of this webpage.

Article Value Rating (This article has been rated by 5 experts)
The value rating has not been started or has been completed.
Comment on This Article
You have to sign in your Newpubli account before you can submit or respond to some comments here.

Please give your comments strictly according to Reader Guide of Newpubli. You should protect properly your private information in your comments. Moreover, your comments should not contain any figures or tables, and should not exceed 3000 characters (including blanks) for each time, but you can submit comments on this article for multiple times with different content.

More comments
  • Comments #3      2016-08-26 23:26:39    
    This manuscript can be 4.
  • Comments #2      2016-08-25 23:25:13    
    the rate of this manuscript can be 5
  • Comments #1      2016-08-24 10:23:09    
    The study has been well designed, and the data they provided is novel and significant. The rate of this preprint manuscript is 4.

Create my account

Forget password?

View & Download
Article Ranks   
Article Metrics   
  • Viewed (1520)
  • Downloaded (82)
Related Literature
Article Sharing

Terms of Use| Privacy Statement| Advertise| Contact us

© Newpubli Corporation unless otherwise stated