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Effect of Low-Concentration of Copper on Virulence Factors of a Pathogenic Escherichia Coli Strain



Author(s)
Yang Yang, Yun Liu, Qiumei Shi, Zhiqiang Zhang, Tonglei Wu, Guoqiang Zhu
Author Affiliation(s)
Jiangsu Co-Innovation Centerfor for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, College of Veterinary Medicine, Yangzhou University, Yangzhou, 225009, China (Yang Yang, Yun Liu, Guoqiang Zhu); Hebei Normal University of Science & Technology, Key Laboratory of Hebei Province Preventive Veterinary Medicine, Qinhuangdao, 066004, China (Qiumei Shi, Zhiqiang Zhang, Tonglei Wu)
Corresponding Author(s)
Guoqiang Zhu (E-mail: yzgqzhu@yzu.edu.cn, yzgqzhu@hotmail.com)
Subject Areas
Bacteriology; Infectious diseases; Inorganic chemistry; Microbiology; Pharmacology; Veterinary medicine


Abstract
Low-concentration dietary copper has been widely used for domestic animals worldwide. To investigate the potential antimicrobial effect of low-concentration of copper, a pathogenic Shiga-like toxigenic Escherichia coli (SLTEC) strain, 107/86, cultured in the medium complemented with 1.0 mmol/L copper, was examined regarding alteration of certain virulence factors. The results showed that, compared to the untreated control strain, the Cu-treated strain 107/86 displayed suppressed growth rate, impaired adherence to IPEC-J2 cells, and decreased stx2e production. Reduced adherence to IPEC-J2 cells was confirmed using a real-time RT-PCR assay which targeted adhesion-associated genes. Additionally, the Cu-treated 107/86 differed from the untreated control with 33-fold lower activity of AI-2 in the quorum sensing system as detected using a luminescence assay. Less biofilm formation and decreased flagella motility halos were detected in the Cu-treated strain. Taken together, low-concentration of copper in the medium likely led to significant attenuation of multiple virulence factors of the SLTEC strain, providing new insights to the antimicrobial effect of copper.


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  • Comments #3      2016-08-26 23:26:39    
    This manuscript can be 4.
  • Comments #2      2016-08-25 23:25:13    
    the rate of this manuscript can be 5
  • Comments #1      2016-08-24 10:23:09    
    The study has been well designed, and the data they provided is novel and significant. The rate of this preprint manuscript is 4.

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