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Performance Comparison of Three Cell Transfection Reagents



Author(s)
Yan-Ying Yuan, Wen-Ming Jiang, Shuo Liu, Suchu Wang, Ying-Xue Sun, Ji-Ming Chen
Author Affiliation(s)
China Animal Health and Epidemiology Center, Qingdao, 266032, China
Corresponding Author(s)
Ji-Ming Chen (E-mail: chenjiming@cahec.cn; jmchen678@qq.com)
Subject Areas
Biochemistry; Genetics; Medical imaging; Molecular & cellular biology


Abstract
Cell transfection has been widely applied in various biomedical fields. Selection of proper cell transfection reagents is critical for the success of cell transfection. Recently some novel cell transfection reagents have been available, but their performance remains unknown. In this study, green fluorescent protein was used as the reporter gene, and 293T cells and chicken embryo fibroblasts were used as the targeted cells, to investigate three cell transfection reagents, classical Lipofectamine 2000, Lipofectamine 3000, and QSI which is produced by a Chinese company. As for 293T cells, on the whole, Lipofectamine 3000 was of the highest transfection efficiency; and QSI was of the lowest transfection efficiency; however, at certain concentrations QSI was also of high transfection efficiency; as for chicken embryo fibroblasts, the three transfection reagents were all of low transfection efficiency without significant difference. The results are of importance for selection of proper cell transfection reagents and relevant studies.


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  • Comments #3      2016-08-26 23:30:59    
    This study is important to evaluate the transfection reagent efficiency in vitro cell culture experiment. This is beneficial to many biology scientists. This can be rated as 3.
  • Comments #2      2016-08-25 23:26:18    
    this manuscript can be rated as 2
  • Comments #1      2016-08-24 10:28:06    
    This study is important for cell biology. Different cell types may be different in respond to different transfection reagents. The authors need test in other cell types as well to reach a conclusion. The value could be 2.

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