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Measurement of the Right Ventricular Systolic Pressure in Rats for Studies of Pulmonary Arterial Hypertension: a Visualized Protocol



Author(s)

Yangbasai Dong, Bing Liu, Hui Gao, Roberto Machado, Ji-Wang Chen

Author Affiliation(s)

Section of Pulmonary, Critical Care, Sleep and Allergy, Department of Medicine, University of Illinois at Chicago, Chicago, IL60612, USA (Yangbasai Dong, Bing Liu, Hui Gao, Roberto Machado, Ji-Wang Chen); Department of Obstetrics and Gynecology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology (HUST), Wuhan 430022, PR China (Hui Gao)
Corresponding Author(s)
Ji-Wang Chen (E-mail: chenjw@uic.edu)
Subject Areas
Anatomy; Cardiology; Clinical examinations; Clinical medicine; Critical care and emergency medicine; Diagnostic medicine; Hematology; Internal medicine; Medical imaging; Medicine; Veterinary medicine; Zoology


Abstract
Pulmonary arterial hypertension (PAH) is a debilitating and fatal disease, often occurring in patients of systemic scleroderma, systemic lupus erythematosus, sickle cell disease, obesity-hypoventilation syndrome, and chronic obstructive pulmonary disease. Rat models are widely used to study the mechanisms and evaluate new therapies of PAH. The models require measurement of rat right ventricular systolic pressure (RVSP) which is technically challenging. Currently no visualized protocol to measure rat RVSP has been reported yet. Here we provide a reproducible visualized workflow for the measurement of RVSP in rats using the Millar system. The measurement includes the steps of tool preparation, catheter calibration, rat anesthetization, external right jugular vein separation, catheter insertion and data recording. Using this protocol, we measured RVSPs in the rat PAH models mediated by hypoxia as well as hypoxia plus sugen. This detailed and visualized protocol for RVSP measurement in rats is useful for the PAH studies.


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  • Comments #1      2016-08-26 23:34:11    
    This is a very useful guidance to all the scientists, who is interested in the research related to the Pulmonary arterial hypertension (PAH). It can be rated as 5.

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